Isiaka Kareem, Mohd Razi Ismail, Adam Puteh Mahamoud Abdillahi Rabileh, Saliu
Adeyemi Kareem, Alasinrin Sikiru Yusuf
Improvement in the yield of rice is a pre-requisite to having selfsufficiency in rice production which has not yet been achieved with pre-germination technology. This study was, therefore, conducted to determine the efficacy of seed priming over pre-germination treatment in improving rice yield. Seed priming with 100mM calcium chloride, 40% (w/v) polyethyl glycol 6000 (PEG6000) and 100ppm kinetin were tested against pre-germination. The experimental design used was randomized complete block design (RCBD) with three replications. The plants were assessed using leaf area index (LAI), absolute growth rate (AGR), relative growth rate (RGR), crop growth rate (CGR), net assimilation rate (NAR), leaf area duration (LAD), plant height, number of tillers, productive tillers, rate of photosynthesis, stomatal conductance, intercellular carbon dioxide, transpiration rate, spikelets per panicle, number of filled spikelets, 100-grain weight, grain yield, harvest index and days to heading. It was found that kinetin priming was 18.24%, 10.32% and 28.31% better in number of spikelets per panicle, grain yield and harvest index respectively than pre-germination that was used as the check. This finding implies that 100ppm kinetin priming could be effectively used for better yield improvement of MR219 rice under optimum conditions and can successfully replace pre-germination with better yield. Read full PDF
Keywords: seed priming, classical growth, gas exchange characteristics, yield, yield attributes
 Itani, T., Tatemoto, H., Okamoto, M., Fujii, K. and Muto, N.( 2002). A comparative study on anti- oxidative activity and polyphenol content of coloured kernel rice. Journal of Japanese Society of Food Science and Technology. 49 (8): 540-543.
 Farooq, M., Basra, S.M.A., Wahid, A. and Khaliq, N.(2009). Kobayashi Rice seed invigoration: a review. In: Lichtfouse, E. (Ed.). Sustainable Agriculture Reviews. 1:137–175.
 Ashraf, M. and Foolad, M.R. (2005). Pre−sowing seed treatment: a shotgun approach to improve germination, plant growth and crop yield under saline and non−saline conditions. Advances in Agronomy. 88: 223−271.
 Ruan, S., Xue, Q. and Tylkowska, K. (2002). The influence of priming on germination of rice (Oryza sativa L.) seeds and seedling emergence and performance in flooded soil. Seed Science & Technology. 30:61-67
 Ghassemi−Golezani, K., Jabbarpour, S., Zehtab−Salmasi, S. and Mohammadi, A. (2010). Response of winter rapeseed (Brassica napus L.) cultivars to salt priming of seeds. African Journal of Agricultural Research. 5: 1089−1094.
 Afzal, S., Akbar, N., Ahmad, Z., Maqsood, Q., Iqbal, M.A. and Aslam, M.R. (2013). Role of Seed Priming with Zinc in Improving the Hybrid Maize (Zea mays L.) Yield. American−Eurasian Journal of Agricultural and Environmental Sciences. 13(3): 301−306.
 Rehman, H., Basra, S.M.A., Farooq, M., Ahmed, N. and Afzal, I. (2011). Seed priming with CaCl2 improves the stand establishment, yield and some quality attributes in direct seeded rice (Oryza sativa). International Journal of Agriculture and Biology. 13: 786–790.
 Hussian, I., Ahmad, R., Farooq, M. and Wahid, A. (2013). Seed Priming improves the performance of poor quality wheat seed. International Journal of Agriculture and Biology. 15: 1343‒1348.
 Ajouri, A., Asgedom, H. and Becker, M. (2004). Seed priming enhances germination and seedling growth of barley under conditions of P and Zn deficiency. Journal of Plant Nutrition and Soil Science 167: 630−636.
Basra, S.M.A., Farooq M., Rehman, H. and Saleem, B.A. (2007). Improving the Germination and Early Seedling Growth in Melon (Cucumismelo L.) by Pre−sowing Salicylicate Treatments. International Journal of Agriculture and Biology. 9(4): 550−554.
Coombs, J., Hind, G., Leegood, R., Tieszen, L. and Vonshak, A. (1985). Analytical techniques. Techniques in Bioproductivity and Photosynthesis. 2: 219-228. Isiaka Kareem et al. / NIPES Journal of Science and Technology Research 2(2) 2020 pp. 59-77 75
Ibrahim, M.H., Jaafar, H.Z., Karimi, E. and Ghasemzadeh, A. (2014). Allocation of secondary metabolites, photosynthetic capacity, and antioxidant activity of Kacip Fatimah (Labisia pumila Benth) in response to and light intensity. The Scientific World Journal. 2014:1-14.
Bates, L., Waldren, R.P. and Teare, I.D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil. 39: 205-207.
Addo-Quaye, A. A., Darkwa, A. A. and Ocloo, G. K. (2011). Growth analysis of component crops in a maizesoybean intercropping system as affected by time of planting and spatial arrangement. ARPN Journal of Agricultural and Biological Science. 6(6): 34-44.
Clawson K.L., Specht, J.E. and Blad B.L. (1986). Growth analysis of soybean isolines differing in pubescence density. Agronomy Journal. 78: 164-172.
Murchie, E. K., Yang, J., Hubbart, S., Horton, P. and Peng, S. (2002). Are there associations between grain filling rate and photosynthesis in the flag leaves of field grown rice? Journal of Experimental Botany. 53: 2217–2224.
Abayomi, Y., George-Arijenja, A. and Kolawole, I. A. (2007). Comparative leaf growth and grain yield responses of hybrid and open-pollinated maize genotypes to nitrogen fertilizer application. Agrosearch. 8(1): 13-26.
Sadeghi, H., Khazaei, F., Yari, L. and Sheidaei, S. (2011). Effect of seed osmopriming on seed germination behavior and vigor of soybean (Glycine max L.). ARPN Journal of Agriculture and Biological Science. 6: 39- 43.
Harris, D., Tripathi, R. S. and Joshi, A. (2002). On-farm seed priming to improve crop establishment and yield in dry direct-seeded rice. Direct seeding: Research Strategies and Opportunities, International Research Institute, Manila, Philippines, 231-240.
Chang-Zheng, H., Jin, H., Zhi-Yu, Z., Song-Lin, R. and Wen-Jian, S. (2002). Effect of seed priming with mixed salt solution on germination and physiological characteristics of seedling in rice (Oryza sativa L.) under stress conditions. Journal of Zhejiang University (Agriculture Life and Science). 28: 175-178.
Dat, J. F., Foyer, C. H. and Scott, I. M. (1998). Changes in salicylic acid and antioxidants during induced thermotolerance in mustard seedlings. Plant Physiology. 188:1455-1461.
Leibfried, A., To, J. P., Busch, W., Stehling, S., Kehle, A., Demar, M., Kieber. J and Lohmann, J. U. (2005). WUSCHEL controls meristem function by direct regulation of cytokinin-inducible response regulators. Nature. 438(7071): 1172-1175.
Sakakibara, H. (2005). Cytokinin biosynthesis and regulation. Vitamins and Hormones. 72:271–87.
Shakirova, F. M., Sakhabutdinova, A. R., Bezrukova, M. V., Fatkhutdinova, R. A. and Fatkhutdinova, D. R. (2003). Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant Science. 164 (3): 317-322.
Werner, T., Motyka, V., Strnad, M. and Schmülling, T. (2001). Regulation of plant growth by cytokinin. Proceedings of the National Academy of Sciences. 98 (18): 10487-10492. Isiaka Kareem et al. / NIPES Journal of Science and Technology Research 2(2) 2020 pp. 59-77 76
Igari, K., Endo, S., Hibara, K. I., Aida, M., Sakakibara, H., Kawasaki, T. and Tasaka, M. (2008). Constitutive activation of a CC‐NB‐LRR protein alters morphogenesis through the cytokinin pathway in Arabidopsis. The Plant Journal. 55 (1): 14-27.
Ranjbarfordoei, A., Samson, R., Van Damme, P. and Lemeur, R. (2001). Effects of drought stress induced by polyethylene glycol on pigment content and photosynthetic gas exchange of Pistacia khinjuk and P. mutica. Photosynthetica. 38 (3): 443-447.
Gauthami, P., Subrahmanyam, D., Padma, V., Rao, P. R. and Voleti, S. R. (2013). Influence of simulated postanthesis water stress on stem dry matter remobilization, yield and its components in rice. Indian Journal of Plant Physiology. 18 (2): 177-182.
Cha-um, S. and Kirdmanee, C.(2008). Effect of osmotic stress on proline accumulation, photosynthetic abilities and growth of sugarcane plantlets (Saccharum officinarum L.). Pakistan Journal of Botany. 40:2541–2552.
Sengupta, S. and Majumder, A.L (2009). Insight into the salt tolerance factors of wild halophytic rice, Porteresia coarctata: A physiological and proteomic approach. Planta. 229: 911–929.
Wankhade, S. D. and Sanz, A. (2013). Chronic mild salinity affects source leaves, physiology and productivity parameters of rice plants (Oryza sativa L., cv. Taipei 309). Plant and Soil. 367 (1-2): 663-672. [
32]Medici, L. O., Azevedo, R. A., Canellas, L. P., Machado, A. T. and Pimentel, C. (2007). Stomatal conductance of maize under water and nitrogen deficits. Pesquisa Agropecuária Brasileira. 42 (4): 599-601.
Chaves, M. M., Flexas, J. and Pinheiro, C. (2009). Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Annals of Botany. 103(4): 551-560.
Flexas, J., Bota, J., Loreto, F., Cornic, G. and Sharkey, T. D. (2004). Diffusive and metabolic limitations to photosynthesis under drought and salinity in C3 plants. Plant Biology. 6 (3): 269-279.
Setter, T. L., Conocono, E. A. and Egdane, J. A. (1996). Possibility of increasing yield potential of rice by reducing panicle height in the canopy. II. Canopy photosynthesis and yield of isogenic lines. Austalian Journal of Plant Physiology. 23: 161–169.
Sheehy, J. E., Dionora, M. J. A. and Mitchell, P. L. (2001). Spikelet numbers, sink size and potential yield in rice. Field Crops Research. 71: 77–85.
Kato, T. (2004). Effect of spikelet removal on grain filling of Akenohoshi, a rice cultivar with numerous spikelets in a panicle. Journal of Agricultural Science. 142: 177–181.
Yang, J., Zhang, J., Huang, Z., Wang, Z., Zhu, Q. and Liu, L. (2002). Correlation of cytokinin levels in the endosperms and roots with cell number and cell division activity during endosperm development in rice. Annals of Botany. 90(3): 369–377.
Umemoto, T., Nakamura, Y. and Ishikura, N. (1994). Effect of grain location on the panicle on activities involved in starch synthesis in rice endosperm. Phytochemistry. 36: 843–847.
Yang, J., Zhang, J., Wang, Z., Liu, K. and Wang, P. (2006). Post-anthesis development of inferior and superior spikelets in rice in relation to abscisic acid and ethylene. Journal of Experimental Botany. 57 (1): 149-160.
Virk, P. S., Khush, G. S. and Peng, S. (2004). Breeding to enhance yield potential of rice at IRRI: the ideotype approach. International Rice Research Notes. 29:5-9. Isiaka Kareem et al. / NIPES Journal of Science and Technology Research 2(2) 2020 pp. 59-77 77
Mohapatra, P. K., Panigrahi, R. and Turner, N. C. (2011). 5 Physiology of spikelet development on the rice panicle: Is manipulation of apical dominance crucial for grain yield improvement? Advances in Agronomy. 110: 333.
Scoefield, G. N., Hirose, T., Gaudron, J. A., Upadhyay, N. M., Ohsugi, R. and Furbank, R. T. (2002). Antisense suppression of the rice transporter gene, OsSUT1, leads to impaired grain filling and germination but does not affect photosynthesis. Functional Plant Biology. 29: 815–826.